Journal: Cell reports
Article Title: Transcriptional programming mediated by the histone demethylase KDM5C regulates dendritic cell population heterogeneity and function
doi: 10.1016/j.celrep.2024.114506
Figure Lengend Snippet: (A) Representative plots and frequencies of splenic XCR1 + cDC1s, XCR1 − CD172a − merocytic DCs (mero), CLEC12A − cDC2A, and CLEC12A + cDC2B of total cDCs (Lin − MHCII + CD11c + CD26 + ) or of total cDC2s (CD172a + ) in female control (top) and Kdm5c ΔItgax (bottom) mice. (B) Representative plots, proportions, and counts of splenic Ly6C + pDCs and Ly6C − pDCs from female control and Kdm5c ΔItgax mice (parent gate: Lin(B220) + SiglecH + CD11c int CD11b − ). (C) BM chimeras were generated in CD45.1 + host mice by reconstituting their BM with a 1:1 mixture of CD45.1 + control and CD45.2 + Kdm5c ΔItgax BM or CD45.2 + Kdm5c ΔItgax BM alone. Proportions of splenic DC subsets are derived from CD45.1 + control and CD45.2 + Kdm5c ΔItgax cells 7 weeks post injection. (D) Proportions of splenic cDCs of mice administered α-IgG2b or α-PDCA1 to deplete pDCs. (E) Frequencies of splenic cDCs from control and Ifnar1 −/− mice. Each symbol represents an individual mouse. Data were pooled from 2–3 experiments of 9–11 mice per group. (A, B, D, and E) Statistical significance was determined by unpaired t test or (C) one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. All error bars represent mean and SEM. See also .
Article Snippet: Anti-mouse CD11c PE/Cy7 (clone N418) , eBioscience , 25-0114-82; RRID:AB_469590.
Techniques: Control, Generated, Derivative Assay, Injection